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The antibody is from purified rabbit serum by affinity purification by way of sequential chromatography on phospho-peptide and non-phospho-peptide affinity columns.
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Overexpression of the gene continues to be observed in many tumors, which leads to chromosome instability, and therefore could contribute to tumorigenesis. This protein was uncovered to affiliate with, and be involved in, the phosphorylation of NPAT protein (nuclear protein mapped to the ATM locus), which participates in mobile-cycle regulated histone gene expression and performs a essential part in advertising mobile-cycle progression in the absence of pRB. Two alternatively spliced transcript variants of the gene, which encode distinct isoforms, are actually explained. Two extra splice variants were being documented but comprehensive nucleotide sequence information and facts is just not however readily available.
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Blocking peptides are peptides that bind exclusively into the goal antibody and block antibody binding. These peptide commonly includes the epitope recognized via the antibody.
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This guide outlines further more doable optimization of cellular and immunoassay parameters to make sure the best possible benefits are...
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This guidebook outlines even more attainable optimization of mobile and immunoassay parameters to guarantee the absolute best effects are...
Antibodies sure to the blocking peptide no more bind to your epitope to the focus on protein. By comparing the staining from your blocked antibody compared to the antibody on your own, one can see which staining is distinct.
Focus on Information Cyclin E belongs to your extremely conserved cyclin family members, whose members are characterised by a extraordinary periodicity in protein abundance in the mobile cycle. Cyclins functionality as regulators of CDK kinases. Distinctive cyclins show distinct expression and degradation designs which add for the temporal coordination of every mitotic read more party. Cyclin E sorts a fancy with and features being a regulatory subunit of CDK2, whose action is necessary for cell cycle G1/S changeover. This protein accumulates with the G1-S stage boundary and is degraded as cells development via S section.
The measurement of protein phosphorylation is a great tool for measuring the modulation of receptor activation by each antibodies...
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